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The Department of Infectious Diseases, Virology at the University Hospital Heidelberg offers a position for a PhD studentworking on Dynamics of HIV-1 post-entry events in the group of Barbara Müller.

 

The interaction of human immunodeficiency virus with its host cell involves numerous highly dynamic steps that are difficult or impossible to investigate by classical approaches. Therefore, our group applies advanced imaging techniques to study dynamic events in HIV-1 assembly at the plasma membrane and in early post-entry stages of HIV-1 replication. In this project, we specifically focus on HIV-1 cell-cell transmission and post-entry stages of HIV-1 replicationwith a focus on dynamic aspects. These processes are complex and require tight regulation in space and time; post-entry events are further influenced by numerous host cell restriction and dependency factors. For their investigation we develop suitable fluorescent virus derivatives and probes using a variety of state of the art fluorescence labeling technologies, and employ advanced fluorescence microscopy approaches, in particular live-cell imaging. Microscopic analyses are complemented by novel biochemical approaches.

 

We offer an interesting, interdisciplinary research topic with biomedical relevance in an interactive scientific environment, including collaborations with national and international partners, at an internationally competitive level. The project is part of SFB1129 and involves close interactions with groups from different disciplines within the SFB. The lab is located in the recently opened Center for Integrative Infectious Disease Research which also houses the state-of-the-art IDIP imaging platform. Candidates have the possibility to apply for the HBIGS International Graduate School to fully benefit from the excellent scientific training of this program.

 

Applicants should have a Master degree (biology, biochemistry, possibly biophysics or molecular medicine). They should be interested in addressing basic virological questions using different methods, with a strong focus on imaging techniques and image analysis. A good background in standard molecular biological methods is expected. Ideal candidates would already have some experience in fluorescence microscopy and image analysis, together with a background in cell biology, biochemistry or biophysics.

 

We are looking forward to meet curious and motivated students who are excited about science. They should enjoy working independently, but also love to interact, discuss and collaborate with scientists from different disciplines and nations.

 

The position is open immediately. Please send your application (CV, academic transcript, motivation letter and contact details of two referees) as a single pdf file to Barbara_mueller@med.uni-heidelberg.de). or apply directly through the HBIGS graduate school Heidelberg (www.hbigs.uni-heidelberg.de).

Deadline for application is May 10, 2019.

 

 

References:

 

Bejarano et al. (2019)HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the Nuclear Pore Complex. Elife 8. pii: e41800. doi: 10.7554/eLife.41800

Sakin et al. (2017). A versatile tool for live-cell imaging and super-resolution nanoscopy studies of HIV-1 Env distribution and mobility. Cell Chem Biol. 24:635-645.e5

Hanne et al. (2016). Stimulated Emission Depletion Nanoscopy Reveals Time-Course of Human Immunodeficiency Virus Proteolytic Maturation. ACS Nano. 10(9):8215-22.

Eckhardt et al. (2011) A SNAP-tagged derivative of HIV-1--a versatile tool to study virus-cell interactions. PLoS One. 2011;6(7):e22007. doi: 10.1371/journal.pone.0022007. Epub 2011 Jul 22.

 

 

Reviews:

Müller et al. (2019). A Spotlight on Viruses - Application of Click Chemistry to Visualize Virus-Cell Interactions. Molecules ;24(3). pii: E481. doi: 10.3390/molecules24030481

Sakin et al. (2016). Labeling of virus components for advanced, quantitative image analyses FEBS L 590:1858-1876

Hanne et al. (2016) Super-resolved insights into human immunodeficiency virus biology. (2016) FEBS Lett. doi: 10.1002/1873-3468.12186

 

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